Search results “Qualitative and quantitative analysis of”
Qualitative vs. Quantitative
Let's go on a journey and look at the basic characteristics of qualitative and quantitative research!
Views: 690253 ChrisFlipp
Qualitative and Quantitative research in hindi  | HMI series
For full course:https://goo.gl/J9Fgo7 HMI notes form : https://goo.gl/forms/W81y9DtAJGModoZF3 Topic wise: HMI(human machine interaction):https://goo.gl/bdZVyu 3 level of processing:https://goo.gl/YDyj1K Fundamental principle of interaction:https://goo.gl/xCqzoL Norman Seven stages of action : https://goo.gl/vdrVFC Human Centric Design : https://goo.gl/Pfikhf Goal directed Design : https://goo.gl/yUtifk Qualitative and Quantitative research:https://goo.gl/a3izUE Interview Techniques for Qualitative Research :https://goo.gl/AYQHhF Gestalt Principles : https://goo.gl/Jto36p GUI ( Graphical user interface ) Full concept : https://goo.gl/2oWqgN Advantages and Disadvantages of Graphical System (GUI) : https://goo.gl/HxiSjR Design an KIOSK:https://goo.gl/Z1eizX Design mobile app and portal sum:https://goo.gl/6nF3UK whatsapp: 7038604912
Views: 46097 Last moment tuitions
Qualitative and Quantitative Data
Quantitative Data vs Qualitative Data Additional Information on Qualitative vs Quantitative Data http://www.moomoomath.com/qualitative-and-quantitative-data.html Data can be divided into two groups called quantitative and qualitative data Quantitative data is numerical Qualitative Data id descriptive data Let’s look at examples of both Examples of quantitative data would be The number of pets, time of day, the temperature outside Quantitative data can be graphed If you count or measure, you are collecting quantitative data There are two types of quantitative data, discrete and continuous Discrete data is usually data you can count and continuous data is usually data you measure. I have a separate video on these two types of data. Qualitative is descriptive or observations and uses words For example, the color of a house, smell of a sock, texture of a shirt Quantitative or Qualitative Consider a cat Quantitative Data would be the cat has 4 legs and weighs 10 pounds Qualitative data would be the cat is yellow, and has soft fur A bookshelf Quantitative would be you have 50 books and is 150 centimeters tall. Qualitative data would be it is multi-color and has a smooth texture You may also enjoy.. Qualitative and Quantitative Data https://www.youtube.com/watch?v=2X-QSU6-hPU Quantitative Qualitative Song https://www.youtube.com/watch?v=-S2EiPD4-W0 -~-~~-~~~-~~-~- Please watch: "Study Skills Teacher's Secret Guide to your Best Grades" https://www.youtube.com/watch?v=f3bsg8gaSbw -~-~~-~~~-~~-~-
Views: 115953 MooMoo Math and Science
Tricks for Qualitative Analysis | Cations | Jee Mains, Advance, NEET, BITSAT and AIIMS
Hello Guys, Memorizing Qualitative analysis isn't a good idea at all. So, IITian explains is going to make it easiest ever. Learn some super crazy tricks for Salt analysis. Qualitative Cation Analysis The composition of relatively complex mixtures of metal ions can be determined using qualitative analysis, a procedure for discovering the identity of metal ions present in the mixture (rather than quantitative information about their amounts). The procedure used to separate and identify more than 20 common metal cations from a single solution consists of selectively precipitating only a few kinds of metal ions at a time under given sets of conditions. Consecutive precipitation steps become progressively less selective until almost all of the metal ions are precipitated. Group 1: Insoluble Chlorides Most metal chloride salts are soluble in water; only Ag+, Pb2+, and Hg22+ form chlorides that precipitate from water. Thus the first step in a qualitative analysis is to add about 6 M HCl, thereby causing AgCl, PbCl2, and/or Hg2Cl2 to precipitate. If no precipitate forms, then these cations are not present in significant amounts. The precipitate can be collected by filtration or centrifugation. Group 2: Acid-Insoluble Sulfides Next, the acidic solution is saturated with H2S gas. Only those metal ions that form very insoluble sulfides, such as As3+, Bi3+, Cd2+, Cu2+, Hg2+, Sb3+, and Sn2+, precipitate as their sulfide salts under these acidic conditions. All others, such as Fe2+ and Zn2+, remain in solution. Once again, the precipitates are collected by filtration or centrifugation. Group 3: Base-Insoluble Sulfides (and Hydroxides) Ammonia or NaOH is now added to the solution until it is basic, and then (NH4)2S is added. This treatment removes any remaining cations that form insoluble hydroxides or sulfides. The divalent metal ions Co2+, Fe2+, Mn2+, Ni2+, and Zn2+ precipitate as their sulfides, and the trivalent metal ions Al3+ and Cr3+ precipitate as their hydroxides: Al(OH)3 and Cr(OH)3. If the mixture contains Fe3+, sulfide reduces the cation to Fe2+, which precipitates as FeS. Group 4: Insoluble Carbonates or Phosphates The next metal ions to be removed from solution are those that form insoluble carbonates and phosphates. When Na2CO3 is added to the basic solution that remains after the precipitated metal ions are removed, insoluble carbonates precipitate and are collected. Alternatively, adding (NH4)2HPO4 causes the same metal ions to precipitate as insoluble phosphates. Group 5: Alkali Metals At this point, we have removed all the metal ions that form water-insoluble chlorides, sulfides, carbonates, or phosphates. The only common ions that might remain are any alkali metals (Li+, Na+, K+, Rb+, and Cs+) and ammonium (NH4+). We now take a second sample from the original solution and add a small amount of NaOH to neutralize the ammonium ion and produce NH3. (We cannot use the same sample we used for the first four groups because we added ammonium to that sample in earlier steps.) Any ammonia produced can be detected by either its odor or a litmus paper test. A flame test on another original sample is used to detect sodium, which produces a characteristic bright yellow color. The other alkali metal ions also give characteristic colors in flame tests, which allows them to be identified if only one is present. Metal ions that precipitate together are separated by various additional techniques, such as forming complex ions, changing the pH of the solution, or increasing the temperature to redissolve some of the solids. For example, the precipitated metal chlorides of group 1 cations, containing Ag+, Pb2+, and Hg22+, are all quite insoluble in water. Because PbCl2 is much more soluble in hot water than are the other two chloride salts, however, adding water to the precipitate and heating the resulting slurry will dissolve any PbCl2 present. Isolating the solution and adding a small amount of Na2CrO4 solution to it will produce a bright yellow precipitate of PbCrO4 if Pb2+ were in the original sample. Summary In qualitative analysis, the identity, not the amount, of metal ions present in a mixture is determined. The technique consists of selectively precipitating only a few kinds of metal ions at a time under given sets of conditions. Consecutive precipitation steps become progressively less selective until almost all the metal ions are precipitated. Other additional steps are needed to separate metal ions that precipitate together. Thanks Team IITian explains ----------------------------------------------------------------------------- Watch our other videos tricks to remember p block reaction https://www.youtube.com/watch?v=GSxtE_nzkyE ------------------------------------------------------------------------------
Views: 103627 IITian explains
Qualitative Versus Quantitative Analysis
Qualitative Versus Quantitative Analysis
Views: 179 Screened-Instructor
Qualitative and Quantitative Analysis: GCSE Chemistry
GCSE Chemistry module: Qualitative and Quantitative By the end of this topic you will have covered: - Chromatography - Test for irons - Titrations GCSE revision videos and apps from LearnersCloud: http://www.learnerscloud.com/student/products/gcse-chemistry To find out more and to start a free trial visit: http://www.learnerscloud.com/student/home/gcse/gcse-revision
Views: 4615 LearnersCloud
Qualitative and Quantitative Methods
Subject: Forensic Science Paper: Instrumental Methods and Analysis
Views: 90 Vidya-mitra
Episode 3: Identifying Qualitative and Quantitative Variables
There are two general types of variables included in statistics: quantitative variables and qualitative variables. In this video we'll define each of these variables and provide examples. Go Premium for only $9.99 a year and access exclusive ad-free videos from Alanis Business Academy. Click here for a 14 day free trial: http://bit.ly/1Iervwb View additional videos from Alanis Business Academy and interact with us on our social media pages: YouTube Channel: http://bit.ly/1kkvZoO Website: http://bit.ly/1ccT2QA Facebook: http://on.fb.me/1cpuBhW Twitter: http://bit.ly/1bY2WFA Google+: http://bit.ly/1kX7s6P Listen to Alanis Business Academy on the go by downloading our new podcast: iTunes: http://bit.ly/1dwKyWi Stitcher: http://bit.ly/PvPjoa Tunein: http://bit.ly/1gLsDH4
Quantitative vs.Qualitative Data
This mini-tutorial will help you understand the differences between qualitative and quantitative forms of data.
Views: 178878 PACE MySPH
Fundamentals of Qualitative Research Methods: Data Analysis (Module 5)
Qualitative research is a strategy for systematic collection, organization, and interpretation of phenomena that are difficult to measure quantitatively. Dr. Leslie Curry leads us through six modules covering essential topics in qualitative research, including what it is qualitative research and how to use the most common methods, in-depth interviews and focus groups. These videos are intended to enhance participants' capacity to conceptualize, design, and conduct qualitative research in the health sciences. Welcome to Module 5. Bradley EH, Curry LA, Devers K. Qualitative data analysis for health services research: Developing taxonomy, themes, and theory. Health Services Research, 2007; 42(4):1758-1772. Learn more about Dr. Leslie Curry http://publichealth.yale.edu/people/leslie_curry.profile Learn more about the Yale Global Health Leadership Institute http://ghli.yale.edu
Views: 146078 YaleUniversity
A Brief Comparison of Qualitative and Quantitative Research Methods
Filmed at the Otaru University of Commerce Sapporo Satellite on May 16, 2012. Taught by Professor Shawn Clankie and assisted by Tatsuya Mima. It is very helpful to use James Neill's handout that can be found at http://wilderdom.com/research/QualitativeVersusQuantitativeResearch.html
Views: 108737 English Lecture
Qualitative Analysis of Amino Acids - Amrita University
▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=63&sim=1094&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Analysis of amino acids is required in several areas of research, and it is a fundamental tool in product analysis. The application imposes different requirements on the analytical method because the amino acids play different roles. Amino acids are the basic constituents of proteins. Qualitative and quantitative analysis of the amino acid composition of hydrolyzed samples of pure proteins or peptides is used to identify the material and to directly measure its concentration Amino acids are also intermediates in metabolic pathways, often not directly involving proteins. The amino acids are measured as elements of physiological and nutritional studies. This has proven particularly important in monitoring the growth of cells in cultures, as used in the production of biopharmaceuticals . Similar considerations lead to the analysis of foods and feeds to ensure that nutritional requirements are met.
Views: 28315 Amrita Vlab
Qualitative Vs Quantitative Research: Difference between them with examples & methods
In this video tutorial, you will get to learn the difference between qualitative and quantitative research, along with the methods and suitable examples. You can further study this topic on our official website:- https://keydifferences.com/difference-between-qualitative-and-quantitative-research.html In keydifferences.com, you can find the substantial differences between two topics.
Views: 66153 Key Differences
Qualitative vs quantitative risk analysis
Confused about ualitative and quantitative risk analysis? Watch the video, find out the difference! www.pmwars.com
Views: 10908 PMwars
3.3 Qualitative and Quantitative Research Design
Do you like this video? Check out full course on Udemy only for 9.99 USD with following link: https://www.udemy.com/research-methods-for-business-students/?couponCode=RESEARCH_METHODS_1
Views: 35974 MeanThat
Quantitative and Qualitative Analysis
Teachers taking action research free online course on WizIQ. Today's class recording is available: http://www.wiziq.com/online-class/2866384-ar-qualitative-and-quantitative-data-analysis My action research project on http://www.amazon.com/Students-Skills-Reading-Comprehension-Tests-ebook/dp/B006JS1MEK
Views: 2850 Nellie Deutsch - Ed.D
Qualitative Vs Quantitative Risk Analysis
This video describes Risk Analysis Techniques - Qualitative and Quantitative. It also describes techniques to do Qualitative and Quantitative Risk Analysis along with developing rating scales. -~-~~-~~~-~~-~- Please watch: "Introduction to MS Paint" https://www.youtube.com/watch?v=fhIGiUWdkzY -~-~~-~~~-~~-~-
Views: 18814 Project Management
Qualitative & Quantitative Analysis Solved Questions -CHEMISTRY
Our aim is to provide quality education free of cost. With this vision, we are providing COMPLETE FREE VIDEO lectures ,for students preparing for IIT JEE,PMT,Class 12th,Class 11th and other classes. Your suggestions are always welcome if any topics ,concepts missed out in our lectures notes. Subscribe our channel update yourself and keep learning !!!!!. Your positive criticisms and valuable suggestions should enable to refine the video lectures further on..
Views: 251 Smart learning
⨘ } Equity Research } Qualitative and Quantitative analysis }
This video lecture discusses about qualitative and quantitative factors, inherent stability and management factors. If you like to have private consultation/lessons, you are very welcome to contact at [email protected] Do not forget to subscribe { Leprofesseur } YouTube channel. Subscription to the channel helps to keep you up-to-date with latest videos. Your feedback and comments are very much appreciated. Sincerely, H.
R For Qualitative Analysis
Introduction to R Tutorial to learn qualitative analysis package Word2Vec. Learn how to turn text into vectors and create a dendrogram and text map of your data. No prior coding experience necessary.
Views: 4338 Eren Kavvas
Qualitative and Quantitative Marketing Research (11:39)
In this video, Dr. Gaurav Bhalla discusses select tools and methods for conducting effective market research. Dr. Bhalla is a faculty member of the University of Maryland’s Master of Professional Studies in Technology Entrepreneurship. For more info, you can visit http://www.mte.umd.edu.
10 Qualitative data analysis
A video tutorial from the National Union of Students, introducing the principles and practice of qualitative data analysis particularly for free text comments on the National Student Survey.
Views: 6644 Kate Little
Differentiate between Qualitative Analysis and Quantitative Analysis
Risk is part and parcel of any project. Risk identification, analysis and management are the foundations of successful project management.Successful project leaders always do two things very well.First they accept and approach a new project with the idea that the project is totally new irrespective of the fact that there could be similarities between earlier projects handled and also the experience base of the team.Second they go through a systematic and through risk analysis of the project as though there is no tomorrow.These qualities enable them to appreciate the project challenges well ahead and they are invariably ready to face the challenges with suitable actions so that the project objectives are achieved as defined in the project charter. Project Management Body of Knowledge (PMBOK) by PMI also brings in very extensively as one of the nine knowledge areas that requires sufficient focus.An analysis of project management literature leads one to conclude that lack of clear risk understanding and management is the reason for the failure of majority of the projects, This is more so for complex and large projects.Risk , by nature ,is difficult for project managers to visualise due to their enthusiasm to go ahead and execute the projects. Also self interest and personal agenda of the project team lead to assessing issues and challenges optimistically rather than realistically.This focus of this web cast will be the understanding of risks from strategic and execution perspectives and dwell of the methodologies for handling the risks.
Views: 5725 TechGig
Qualitative and Quantitative Risk Analysis
Project Management Assignment 1
Views: 1731 syahirah shaari
Qualitative and Quantitative Analysis of Antibody Drug Conjugates in Preclinical Plasma Samples
Thermo Fisher Scientific poster presentation at AAPS 2016 on Robust and Sensitive Workflow Solution for Qualitative and Quantitative Analysis of Antibody Drug Conjugates in Preclinical Plasma Samples
GPR: Qualitative to Quantitative Analysis - Peter Annan, Sensors and Software
Discussion of the progression from qualitative to quantitative analysis of GPR data, Peter Annan (Sensors and Software). This talk is presented as part of the Agricultural Geophysics Webinar series (ag-geophysics.org). Join the live discussion on Sept.30, 2014 at 3:00PM (EST). Register for the discussion in advance at ag-geophysics.org as space is limited!
Views: 3217 AgGeophysics
Quantitative and Qualitative Risk Assessment - CompTIA Security+ SY0-401: 2.1
Security+ Training Course Index: http://professormesser.link/sy0401 Professor Messer’s Course Notes: http://professormesser.link/sy0401cn Frequently Asked Questions: http://professormesser.link/faq - - - - - Risk factors can take many forms. In this video, you’ll learn how to asses both quantitative and qualitative risk factors in your environment. - - - - - Download entire video course: http://professormesser.link/401adyt Get the course on MP3 audio: http://professormesser.link/401vdyt Subscribe to get the latest videos: http://professormesser.link/yt Calendar of live events: http://www.professormesser.com/calendar/ FOLLOW PROFESSOR MESSER: Professor Messer official website: http://www.professormesser.com/ Twitter: http://www.professormesser.com/twitter Facebook: http://www.professormesser.com/facebook Instagram: http://www.professormesser.com/instagram Google +: http://www.professormesser.com/googleplus
Views: 61199 Professor Messer
Qualitative and Quantitative Analysis
A qualitative and quantitative analysis of a forehand topspin serve
Views: 948 iheromusic
PMP® | Risk Analysis-Qualitative & Quantitative Approaches
Stuck with PMP? Enroll in our PMP Online Training and Get PMP in 45 Days with PMBOK5 http://goo.gl/l2aQOq Agenda for the hangout Check out the queries raised at iZenBridge Forum http://goo.gl/pVUqF7 to be discussed during the session.  https://plus.google.com/b/103624728377270787243/events/cbo8qceqi7v7a344311id8rp3ts Our Trainers will focus on the queries posted by participants for discussion in the hangout. https://twitter.com/iZenBridge You can also post your queries at the forum. How to join the hangout? You can watch the event live at Risk Analysis Hangout. The queries/ questions will be taken up by using Q & A app.during the hangout. You need to type in your questions just like before, but now, the Q & A app will make it more manageable for the participants. Only selected participants, who have raised queries in the forum, would get an opportunity to come inside the session by way of video to follow up on the queries they had raised. Only first 9 participants can avail this facility. To enter the live web session, the selected participants can use the link provided at Twitter. Search the link using #iZenPMP. Rest of the participants need to follow the link provided above in the mail. Only in case of technical problems during the hangout, communicate to us on Twitter by using #iZenPMP Speaker:+Saket Bansal
Project Management Concept #2: Qualitative Risk Analysis vs Quantitative Risk Analysis
Passionate Project Management, www.PassionatePM.com, is pleased to sponsor this Passionate Project Management YouTube channel. Passionate Project Management specializes in project management learning, development, and consulting. Passionate Project Management offers open enrollment and private on-site courses delivered by the leading instructors in the industry. Passionate Project Management provides PMP Exam Prep, CAPM Exam Prep, PMI-RMP (Risk Management) Exam Prep, and PMI-ACP (Agile) Exam prep. This Video is a component of our Learning Concepts Series: 2. Qualitative versus Quantitative Risk Analysis narrated by Belinda Fremouw. Belinda was the first woman in the world to achieve the original five PMI Credentials. Her experience spans multiple decades, crosses several industries, and includes domestic and international project experience. Belinda is a well-known public speaker and author. Check out our website at www.PassionatePM.com for all of our course offerings and exam prep study materials. www.PassionatePM.com #PassionatePM #BelindaFremouw #BelindaPMP
Views: 21287 PM Learning Solutions
Quantitative vs Qualitative Research
ETSU EDFN 5950 midterm submission
Views: 135937 smcilquham
Qualitative & Quantitative Research - An Introduction
An introduction to the subjects of Qualitative and Quantitative research. When organisations enter into strategic planning, they often conduct different types of relevant research and analyses enabling them to make informed, strategic decisions. Parts of this process might include conducting qualitative and/or quantitative research, which is what this video aims to explain and exemplify. This video is aimed at marketing management students.
Views: 55473 Tine Wade
Complete video lectures are available in DVD & USB. Payment can be made by debit card/credit card/COD. Cash on Delivery is also available Click to buy courses http://www.nucleoniitjeekota.com These videos are classroom recording of lecture taken at Nucleon chemistry classes kota . Nucleon is the individual institute of kota dedicated for IIT-JEE Advanced chemistry . Nucleon is run by two subject experts NV sir (B.Tech. IIT Delhi ) & VKP sir (M. Sc. IT BHU ). Qualitative analysis Lecture -1 Preliminary test, Dry Heating test, Flame test, Borax bead test, Charcoal cavity test. Cobalt nitrate test Lecture -2 Group A anion, radial Lecture -3, , s 2– Lecture -4 starch / iodide test, Brown ring test, CH 3 COO – , Lecture -5 , Iodometry test Lecture -6 Cl – , F – , Br – , I – Lecture -7 Nitrate,oxalate, borate ion Lecture -8 Group B anion Sulphate,phosphate,permanganate,chromate anion Lecture -9 Cation (basic radical) Zero group – Ist group – Pb +2 Lecture -10 Ist group – Ag + , Hg +2 Lecture -11 II A group – IIA & IIB IIA – Cu +2 , Bi +3 , Pb +2 , Cd +2 , Hg +2 Lecture -12 III group – Al +3 , Fe +3 , Cr +3 IV group – Mn +2 Lecture -13 V group – Ba +2 , Sr +2 , Ca +2 VI group – Mg +2
Learning Qualitative Analysis in Chemistry using Mnemonics i.e  Easy Memory Recall Tips and Symbols
This video will not only show you how to learn qualitative analysis in chemistry but will also show you how to recall what you have learnt with ease. It is recommended for all chemistry students in senior secondary schools.
Empirical Studies: Qualitative vs. Quantitative
This video walks you through the differences between quantitative and qualitative research methods.
Views: 1814 USU Libraries
Types of Fundamental Analysis - Quantitative and Qualitative (HINDI)
Types of Fundamental Analysis is mistaken as only quantitative analysis. However, there are two Types of Fundamental Analysis i.e. qualitative and quantitative fundamental analysis. Sometimes viewers post a query despite buying a fundamentally strong stock, they are in loss. The answer is that a stock might be strong on one of the fundamental analysis i.e. quantitative but might be very weak in qualitative analysis. Quantitative analysis is done with the help of financial statements, balance sheet etc. On the other hand, qualitative analysis is done with the help of tools like SWOT Analysis. Out of two Types of Fundamental Analysis, qualitative analysis is more imp compared to quantitative. The reason being, qualitative fundamental analysis tells why the stock or company is great and whether it will remain great in future or not. It also helps to find out the competitive advantage of the company compared to its peers. Competitive advantage is also called MOAT. To summarize, both Types of Fundamental Analysis is imp as numbers alone are incomplete without qualitative analysis. If you liked this video, You can "Subscribe" to my YouTube Channel. The link is as follows https://goo.gl/nsh0Oh By subscribing, You can daily watch a new Educational and Informative video in your own Hindi language. For more such interesting and informative content, join me at: Website: http://www.nitinbhatia.in/ T: http://twitter.com/nitinbhatia121 G+: https://plus.google.com/+NitinBhatia #NitinBhatia
Views: 14611 Nitin Bhatia
Quantitative and Qualitative Research
This video describes and compares the characteristics of quantitative and qualitative research in counseling.
Views: 20719 Dr. Todd Grande
Semi quantitative, quantitative and qualitative
Semi quantitative, quantitative and qualitative
Views: 744 Rachna Sharma
Phenomenology vs. empirics? From qualitative to quantitative analysis of literature - and back
International Conference BRIDGING GAPS, CREATING LINKS: Qualitative and Quantitative Approaches to the Study of Literature Dept. of Linguistic and Literary Studies University of Padua, Italy 7-9 June 2018 Phenomenology vs. empirics? From qualitative to quantitative analysis of literature - and back JAN CHRISTOPH MEISTER (University of Hamburg)
01.Organic Chemistry_ Introduction: 05 - Qualitative and Quantitative Analysis of Organic compounds
Hi All, Welcome back to MedicoPharma Lectures once again. This lecture is going to discuss the qualitative and quantitative analysis of organic compounds. This lecture covers detection of carbon, detection of hydrogen, detection of nitrogen, sulphur and Halogen in Organic Compounds. It also includes preparation of sodium fusion extract. This video is very very useful for entrance exam preparation. It also helps in conducting organic practical classes. For better understanding, kindly see our previous lectures on organic chemistry. Kindly provide your feedback for our improvement...!!! Thanks for watching us.
Qualitative and Quantitative Methods
Richard Garfield explains when qualitative and quantitative assessment methods should be used.
Views: 4599 ACAPS Project
Views: 15698 CHEMISTRY by BKM SIR
Qualitative and Quantitative Analysis (Chandelier) - A&A
This music video is part of a video presentation/ reporting for the topic of Qualitative and Quantitative Analysis in the Industry under the subject Fundamentals of Industrial Engineering and Management (IEM632; Masters of Science in Industrial Engineering and Management- Polytechnic University of the Philippines)
Views: 102 Alexis Carpio
Application of light sheet microscopy for qualitative and quantitative analysis of bronchus
Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice. David Twapokera Mzinza et al (2018), Cellular and Molecular Immunology https://doi.org/10.1038/cmi.2017.150 Bronchus-associated lymphoid tissue (BALT) develops at unpredictable locations around lung bronchi following pulmonary inflammation. The formation and composition of BALT have primarily been investigated by immunohistology that, due to the size of the invested organ, is usually restricted to a limited number of histological sections. To assess the entire BALT of the lung, other approaches are urgently needed. Here, we introduce a novel light sheet microscopy-based approach for assessing lymphoid tissue in the lung. Using antibody staining of whole lung lobes and optical clearing by organic solvents, we present a method that allows in-depth visualization of the entire bronchial tree, the lymphatic vasculature and the immune cell composition of the induced BALT. Furthermore, three-dimensional analysis of the entire lung allows the qualitative and quantitative enumeration of the induced BALT. Using this approach, we show that a single intranasal application of the replication-deficient poxvirus MVA induces BALT that constitutes up to 8% of the entire lung volume in mice deficient in CCR7, in contrast to wild type mice (WT). Furthermore, BALT induced by heat-inactivated E. coli is dominated by a pronounced T cell infiltration in Cxcr5-deficient mice, in contrast to WT mice.
Views: 30 ScienceVio

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